The emergence of plasmid-mediated colistin resistance genes (mcr), which is occurring in numerous countries, is a worldwide concern, primarily because colistin is a last-resort antibiotic. […]
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Comparison of RNA isolation methods on RNA-SEQ: Implications for differential expression and meta-analysis.
The increasing number of transcriptom datasets has enabled meta-analysis, which can be valuable because of the increase in their statistical strength. However, the meta-analysis can […]
Continue readingSmall RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue
Because of the important role in gene regulation, non-default RNA (SRNA) of 20-30 nucleotides (NT) has been studied intensively in mammals and plants and is […]
Continue readingAn optimized step-by-step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse intervertebral discs and subsequent preparation of high-quality intact total RNA
The degeneration of intervertebral disk is the most significant, and least understood, causing chronic back pain, which affects almost one of seven individuals at a […]
Continue readingIsolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping
Visceral Adipose Tissue (VAT) is an active metabolic organ that consists mainly of mature adipocytes and cells of the vascular fraction (SVF), which releases different […]
Continue readingRNA Isolation from Articular Cartilage Tissue
High quality RNA isolation directly from the desired network to get the right information from the profiles of gene in vivo expression in cells embedded […]
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